Journal: Journal of Cardiovascular Translational Research
Article Title: Extension of Atherosclerosis ApoE-/- Mouse—a Model of Chronic Myocardial Ischemia and Evaluation Method
doi: 10.1007/s12265-025-10734-8
Figure Lengend Snippet: A HE: It can be seen that the cardiomyocytes in the model group are significantly enlarged and the nuclei become sparse. Sirius red staining: Some areas of myocardium in the model group showed an increase in cell spacing accompanied by fibrotic tissue metaplasia. In contrast, myocytes in the control group were neatly arranged and densely distributed, with significantly less fibrous tissue than in the model group. “LV”: left ventricle. “RV”: right ventricle. B In the model group, a small number of apoptotic cells were observed with green fluorescence, but no large aggregates were detected. C Additionally, there were numerous CD68-positive cells expressing red fluorescence, which were irregularly distributed on the cell wall and in the cytoplasm
Article Snippet: Tissue sections underwent blocking with 5% bovine serum albumin (BSA) (Sigma-Aldrich Cat# A7906) in PBST (0.1% Tween-20 in PBS) for 1 h at room temperature, followed by overnight incubation at 4 °C with anti-CD68 primary antibody (Rabbit polyclonal, Bioss Antibodies, Cat# bs-20403R, RRID: AB_10855800) diluted 1:100 in blocking solution.
Techniques: Staining, Control, Fluorescence, Expressing